Journal: Current protocols in chemical biology

Article Title: A Detailed Guide for Metabolic Incorporation of N -acetyl muramic Acid Probes into Bacterial Peptidoglycan

doi: 10.1002/cpch.74

Figure Lengend Snippet: Troubleshooting the Bacterial Cell Wall Labeling (Basic Protocol 4)

Article Snippet: Materials Stock solutions of sugars (0.1 M of NAM, 2-AzNAM, 2-AlkNAM, or 3-AzNAM in Milli-Q ® water) E. coli ΔMurQ-KU cells (see Liang, et. al 2017 2017 for strain construction; for access to strain, contact corresponding author) Petri Dishes with Clear Lid (Fisher- Catalog ID FB0875712) Isopropyl-1-thio-β-D-galactoside (IPTG) (Gold Biotechnology-Catalog ID I2481) Lysogeny broth (LB) Bacto Agar (Fisher) Chloramphenicol (34 mg/mL stock in Milli-Q ® water) (Gold Biotechnology-Catalog ID C-105) Kanamycin (50 mg/mL stock in Milli-Q® water) (Gold Biotechnology-Catalog ID K-120) Fosfomycin (10 mg/mL in Milli-Q® water) (Gold Biotechnology- Catalog ID F-470) 1 x Phosphate buffered saline (PBS) Copper sulfate (CuSO 4 ) (Fisher) Freshly prepared (+)− sodium (L) ascorbate (Fisher) Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (Sigma) Fluor 488-Alkyne (Sigma- Catalog ID61621) Poly-L-lysine (Sigma) Milli-Q ® water Cover glasses (Zeiss) 1.5 mL sterile Eppendorf tubes Prolong Diamond Antifade Mountant (Invitrogen) Sterile culture tubes 37 °C incubator Zeiss Elyra PS.1 microscope with Plan-Apochromat × 63/1.4 Oil differential interference contrast (DIC) M27 objective Plate shaker UV/Vis Spectrophotometer Tweezers Preparation of Stock Bacterial Plate 1.

Techniques: Labeling, Fluorescence, Concentration Assay, Imaging

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.

doi: 10.1016/j.jchromb.2017.07.036

Figure Lengend Snippet: Fig. 1. (a) Chemical structure of fosfomycin and (b) the internal standard: fosfomycin- 13C3. Only one of the enantiomers from the racemic mixture is depicted. The three 13C atoms are marked as C*.

Article Snippet: Fosfomycin was purchased from Santa Cruz Biotechnology Inc. (Huissen, the Netherlands, purity> 98%) and racemic fosfomycin-13C3 benzylamine salt, which was used as the internal standard, was purchased from Toronto Research Chemicals (North York, Canada, purity 96%).

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.

doi: 10.1016/j.jchromb.2017.07.036

Figure Lengend Snippet: Fig. 2. Chromatograms of fosfomycin in urine (a) and plasma (b) and the internal standard in urine (c) and in plasma (d) after injection of calibration standard 3.

Article Snippet: Fosfomycin was purchased from Santa Cruz Biotechnology Inc. (Huissen, the Netherlands, purity> 98%) and racemic fosfomycin-13C3 benzylamine salt, which was used as the internal standard, was purchased from Toronto Research Chemicals (North York, Canada, purity 96%).

Techniques: Clinical Proteomics, Injection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The Combination of Fosfomycin plus Meropenem Is Synergistic for Pseudomonas aeruginosa PAO1 in a Hollow-Fiber Infection Model

doi: 10.1128/AAC.01682-18

Figure Lengend Snippet: Monotherapy evaluations of fosfomycin and meropenem against P. aeruginosa PAO1 in the HFIM. Panels A to E.

Article Snippet: Fosfomycin disodium salt (fosfomycin for injection, Zolyd; Zavante Therapeutics, recently acquired by Nabriva Therapeutics) was used for the susceptibility testing, preparation of resistance-selecting plates, bioanalytical methods (liquid chromatography/tandem mass spectrometry [LC-MS/MS]) and as medium for the hollow-fiber infection model studies.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The Combination of Fosfomycin plus Meropenem Is Synergistic for Pseudomonas aeruginosa PAO1 in a Hollow-Fiber Infection Model

doi: 10.1128/AAC.01682-18

Figure Lengend Snippet: Combination therapy with fosfomycin and meropenem against P. aeruginosa PAO1. Panels F to I.

Article Snippet: Fosfomycin disodium salt (fosfomycin for injection, Zolyd; Zavante Therapeutics, recently acquired by Nabriva Therapeutics) was used for the susceptibility testing, preparation of resistance-selecting plates, bioanalytical methods (liquid chromatography/tandem mass spectrometry [LC-MS/MS]) and as medium for the hollow-fiber infection model studies.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The Combination of Fosfomycin plus Meropenem Is Synergistic for Pseudomonas aeruginosa PAO1 in a Hollow-Fiber Infection Model

doi: 10.1128/AAC.01682-18

Figure Lengend Snippet: Parameter estimates for all parameters for the combination of meropenem plus fosfomycin against Pseudomonas aeruginosa

Article Snippet: Fosfomycin disodium salt (fosfomycin for injection, Zolyd; Zavante Therapeutics, recently acquired by Nabriva Therapeutics) was used for the susceptibility testing, preparation of resistance-selecting plates, bioanalytical methods (liquid chromatography/tandem mass spectrometry [LC-MS/MS]) and as medium for the hollow-fiber infection model studies.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: The Combination of Fosfomycin plus Meropenem Is Synergistic for Pseudomonas aeruginosa PAO1 in a Hollow-Fiber Infection Model

doi: 10.1128/AAC.01682-18

Figure Lengend Snippet: Predicted-observed regressions for pre-Bayesian (population) and Bayesian (individual) analyses for (A) meropenem concentrations, (B) fosfomycin concentrations, (C) total bacterial burden, (D) meropenem-resistant bacterial burden, and (E) fosfomycin-resistant bacterial burden.

Article Snippet: Fosfomycin disodium salt (fosfomycin for injection, Zolyd; Zavante Therapeutics, recently acquired by Nabriva Therapeutics) was used for the susceptibility testing, preparation of resistance-selecting plates, bioanalytical methods (liquid chromatography/tandem mass spectrometry [LC-MS/MS]) and as medium for the hollow-fiber infection model studies.

Techniques: